                         SEQUENCE LISTING

<110>  INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND 
       BIOTECHNOLOGY
 
<120>  A NOVEL DENGUE ENVELOPE DOMAIN III-BASED TETRAVALENT PROTEIN 
       VACCINE

<130>  IN:1259-DEL-2007

<140>  1259-DEL-2007
<141>  2007-06-12

<160>  2     

<170>  PatentIn version 3.3

<210>  1
<211>  499
<212>  PRT
<213>  Artificial

<220>
<223>  This protein sequence is encoded by a polynucleotide sequence 
       having SEQ ID No.: 2


<220>
<221>  OCH
<222>  (499)..(499)
<223>  X at position 499 is OCH group

<400>  1

Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 
1               5                   10                  15      


Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly 
            20                  25                  30          


Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 
        35                  40                  45              


Val Thr Gln Asn Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp Lys 
    50                  55                  60                  


Lys Pro Val Asn Ile Glu Thr Glu Pro Pro Phe Gly Glu Ser Tyr Ile 
65                  70                  75                  80  


Val Val Gly Ala Gly Glu Lys Ala Lys Gln Trp Phe Lys Lys Gly Ser 
                85                  90                  95      


Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly Ala Arg Arg Met 
            100                 105                 110         


Ala Ile Leu Gly Gly Gly Gly Gly Met Ser Tyr Ala Met Cys Leu Asn 
        115                 120                 125             


Thr Phe Val Leu Lys Lys Glu Val Ser Glu Thr Gln His Gly Thr Ile 
    130                 135                 140                 


Leu Ile Lys Val Glu Tyr Lys Gly Glu Asp Ala Pro Cys Lys Ile Pro 
145                 150                 155                 160 


Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala His Asn Gly Arg Leu Ile 
                165                 170                 175     


Thr Ala Asn Pro Val Val Thr Lys Lys Glu Glu Pro Val Asn Ile Glu 
            180                 185                 190         


Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile Val Ile Gly Ile Gly Asp 
        195                 200                 205             


Lys Ala Leu Lys Ile Asn Trp Tyr Arg Lys Gly Ser Ser Ile Gly Lys 
    210                 215                 220                 


Met Phe Glu Ala Thr Ala Arg Gly Ala Arg Arg Met Ala Ile Leu Gly 
225                 230                 235                 240 


Gly Gly Gly Gly Met Ser Tyr Met Cys Gly Lys Phe Ser Gly Lys Phe 
                245                 250                 255     


Ser Ile Asp Lys Glu Met Arg Glu Thr Gln His Gly Thr Thr Val Val 
            260                 265                 270         


Lys Val Lys Tyr Glu Gly Ala Gly Ala Pro Cys Lys Val Pro Ile Glu 
        275                 280                 285             


Ile Arg Asp Val Asn Lys Glu Lys Val Val Gly Arg Ile Ile Ser Ser 
    290                 295                 300                 


Thr Pro Leu Ala Glu Asn Thr Asn Ser Val Thr Asn Ile Glu Leu Glu 
305                 310                 315                 320 


Arg Pro Leu Asp Ser Tyr Ile Val Ile Gly Val Gly Asn Ala Leu Thr 
                325                 330                 335     


Leu His Trp Phe Arg Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ser 
            340                 345                 350         


Thr Tyr Arg Gly Ala Lys Arg Met Ala Ile Leu Gly Gly Gly Gly Gly 
        355                 360                 365             


Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 
    370                 375                 380                 


Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 
385                 390                 395                 400 


Asp Gly Ser Pro Cys Lys Thr Pro Phe Glu Ile Met Asp Leu Glu Lys 
                405                 410                 415     


Arg His Val Leu Gly Arg Glu Thr Thr Val Asn Pro Ile Val Thr Glu 
            420                 425                 430         


Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 
        435                 440                 445             


Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asp Trp Phe 
    450                 455                 460                 


Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 
465                 470                 475                 480 


Ala Lys Arg Met Ala Ile Leu Gly Gly Gly Gly Gly His His His His 
                485                 490                 495     


His His Xaa 
            


<210>  2
<211>  1512
<212>  DNA
<213>  Artificial

<220>
<223>  This polynucleotide sequence is derived from dengue virus 
       serotypes, DEN-1, DEN-2, DEN-3 and DEN-4

<400>  2
gaattcacta tgtcctacgt tatgtgtact ggttccttca agttggagaa ggaagttgct     60

gaaactcagc acggtactgt tttggttcag gttaagtacg aaggtactga cgctccatgt    120

aagatcccat tctccactca agatgagaag ggtgttactc agaacagatt gatcactgct    180

aacccaatcg ttactgacaa gaagccagtt aacatcgaaa ctgagccacc attcggtgaa    240

tcctacatcg ttgttggtgc tggtgaaaag gctaagcagt ggttcaagaa gggttcctcc    300

atcggtaaga tgttcgaggc tactgctaga ggtgctagaa gaatggctat cttgggtggt    360

ggtggtggaa tgtcttacgc tatgtgtttg aacactttcg ttttgaagaa ggaggtttcc    420

gagactcaac acggtactat cttgatcaag gttgagtaca agggtgaaga tgctccttgt    480

aagattccat tctcaactga ggacggtcaa ggtaaggctc ataacggtag attgattaca    540

gctaatccag ttgttactaa gaaggaggag cctgttaaca ttgaagctga accacctttc    600

ggagagtcca acatcgttat cggtatcggt gacaaggctt tgaagatcaa ctggtacaga    660

aagggatctt ctattggaaa gatgtttgaa gctacagcta gaggagctag aagaatggct    720

attttgggag gtggtggagg aatgtcctac atgtgtggta agttctccgg aaaattctcc    780

attgacaagg agatgagaga gactcagcac ggaactacag ttgttaaggt taagtatgag    840

ggtgctggtg ctccatgtaa agttcctatc gagatcagag atgttaacaa ggagaaagtt    900

gttggtagaa tcatctcctc cactccattg gctgaaaaca ctaactccgt tacaaacatc    960

gagttggaga gaccattgga ctcctacatt gttatcggtg ttggtaacgc tttgactttg   1020

cactggttta gaaagggatc atcaatcgga aaaatgttcg agtccactta cagaggtgct   1080

aagagaatgg ctatcttggg aggtggtgga ggaatgtctt actccatgtg tactggaaag   1140

ttcaaagttg ttaaggagat cgctgagaca cagcatggta ctatcgttat cagagttcag   1200

tacgagggtg atggttcccc ttgtaagact ccattcgaga tcatggactt ggagaagaga   1260

cacgttttgg gaagattgac tactgttaac cctattgtta cagagaagga ctcccctgtt   1320

aatatcgagg ctgagcctcc atttggtgac tcttacatca tcatcggtgt tgagcctggt   1380

caattgaagt tggactggtt taagaaagga tcttccattg gtcaaatgtt cgagactact   1440

atgagaggag ctaagagaat ggctattttg ggtggaggag gtggacatca tcaccatcac   1500

cactaagaat tc                                                       1512


